Selected Recent Publications


Helminth extracellular vesicles: Great balls of wonder

Hoffmann, KF, Hokke, CH, Loukas, A & Buck, AH 2020, In : International Journal for Parasitology, vol. 50, no. 9, pp. 621-622.

Article DOI:


Pilot Evaluation of Two Fasciola hepatica Biomarkers for Supporting Triclabendazole (TCBZ) Efficacy Diagnostics

Collett, C., Morphew, R., Timson, D., Phillips, H. & Brophy, P., 30 Jul 2020, In : Molecules. 25, 15, 3477.

Article DOI:


Novel Equine Faecal Egg Diagnostics: Validation of the FECPAKG2

Tyson, F., Dalesman, S., Brophy, P. & Morphew, R., 23 Jul 2020, In : Animals. 10, 8, 10 p., 1254.

Article DOI:


DC-SIGN mediated internalisation of glycosylated extracellular vesicles from Schistosoma mansoni increases activation of monocyte-derived dendritic cells

Kuipers, ME, Nolte-‘t Hoen, ENM, van der Ham, AJ, Ozir-Fazalalikhan, A, Nguyen, DL, de Korne, CM, Koning, RI, Tomes, JJ, Hoffmann, KF, Smits, HH & Hokke, CH In: Journal of Extracellular Vesicles, vol. 9, no. 1, 1753420.

Article DOI:



The repositioning of epigenetic probes/inhibitors identifies new anti-schistosomal lead compounds and chemotherapeutic targets.

Whatley KCL, Padalino G, Whiteland H, Geyer KK, Hulme BJ, Chalmers IW, Forde-Thomas J, Ferla S, Brancale A, Hoffmann KF. In: PLoS Negl Trop Dis. 2019 Nov 15;13(11):e0007693.

Article DOI: 10.1371/journal.pntd.0007693


Antischistosomal Properties of Sclareol and Its Heck-Coupled Derivatives: Design, Synthesis, Biological Evaluation and Untargeted Metabolomics

Crusco, A, Whiteland, H, Baptista, R, Forde-Thomas, JE, Beckmann, M, Mur, LAJ, Nash, RJ, Westwell, AD & Hoffmann, KF P. 2019, In ACS Infectious Diseases.

Article DOI:


Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

Wannaporn, I, Mann, V, Karinshak, S, Coghlan, A, Rinaldi, G, Sankaranarayanan, G, Chaidee, A, Tanno, T, Kumkhaek, C, Prangtaworn, P, Mentink-Kane, MM, Cochran, CJ, Driguez, P, Holroyd, N, Tracey, A, Rodpai, R, Everts, B, Hokke, CH, Hoffmann, K, Berriman, M & Brindley, PJ 2019, ' eLife, vol. 8, e41337.

Article DOI:


Schistosoma mansoni venom allergen-like proteins: phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity

Farias, LP, Chalmers, IW, Perally, S, Rofatto, HK, Jackson, CJ, Brown, M, Khouri, MI, Barbosa, MMF, Hensbergen, PJ, Hokke, CH, Leite, LCC & Hoffmann, KF 2019 International Journal for Parasitology.

Article DOI:


The anti-mycobacterial activity of a diterpenoid-like molecule operates through nitrogen and amino acid starvation

Crusco, A, Alves Baptista, R, Bhowmick, S, Beckmann, M, Mur, L, Westwell, AD & Hoffmann, K 2019, ' Frontiers in Microbiology, vol. 10, 01444


Article DOI:

The importance of extracellular vesicle purification for downstream analysis: A comparison of differential centrifugation and size exclusion chromatography for helminth pathogens.

Davis CN, Phillips H, Tomes JJ, Swain MT, Wilkinson TJ, Brophy PM, Morphew, RM. In: PLoS Negl Trop Dis. 2019 Feb 27;13(2):e0007191

Article DOI: 10.1371/journal.pntd.0007191


Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins.

Pawluk, R. J., Stuart, R., de Leaniz, C. G., Cable, J., Morphew, R. M., Brophy, P. M. and Consuegra S. 2019 In: Journal of Proteome Research.

Article DOI: 10.1021/acs.jproteome.8b00953



Polyomic tools for an emerging livestock parasite, the rumen fluke Calicophoron daubneyi; identifying shifts in rumen functionality

Huson, K. M., Morphew, R., Allen, N., Hegarty, M., Worgan, H., Girdwood, S., Jones, E. L., Phillips, H., Vickers, M., Swain, M., Smith, D., Kingston-Smith, A. & Brophy, P. 2018 In : Parasites & Vectors.11, 1, 22 p., 617

Article DOI: 10.1186/s13071-018-3225-6


Combining bioinformatics, cheminformatics, functional genomics and whole organism approaches for identifying epigenetic drug targets in Schistosoma mansoni

Padalino, G., Ferla, S., Brancale, A., Chalmers, I. & Hoffmann, K. 2018 In : International Journal for Parasitology: Drugs and Drug Resistance.

Article DOI: 10.1016/j.ijpddr.2018.10.005. Epub 2018 Nov 13


Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Théry et al. 2018 In : Journal of Extracellular Vesicles

Article DOI: 10.1080/20013078.2018.1535750


An Abeis procera - Derived tetracyclic triterpene containing a steroid-like core and a lactone side chain attenuates in vitro survival of both Fasciola hepatica and Schistosoma mansoni

Whiteland, H., Chakroborty, A., Forde-Thomas, J., Crusco, A., Cookson, A., Hollinshead, J., Fenn, C., Bartholomew, B., Holdsworth, P. A., Fisher, M., Nash, R. J. & Hoffmann, K. 2018 In : International Journal for Parasitology: Drugs and Drug Resistance

Article DOI:  10.1016/j.ijpddr.2018.10.009


Antibody Responses to Schistosoma mansoni Schistosomula Antigens

Egesa M., Lubyayi L, Jones F, van Diepen A, Chalmers I, Tukahebwa E, Bagaya B, Hokke C, Hoffmann K, Dunne D, Elliott A,8, Yazdanbakhsh M, Wilson S, Cose S.  21 Sep 2018 In : Parasite Immunology. e12591.

Article DOI: 10.1111/pim.12591


S. mansoni Schistosomula Antigens Induce Th1/Proinflammatory Cytokine Responses

Egesa, M., Lubyayi, L., Tukahebwa, E. M., Bagaya, B. S., Chalmers, I., Wilson, S., Hokke, C. H., Hoffmann, K., Dunne, D. W., Yazdanbakhsh, M., Labuda, L. A. & Cose, S. 21 Sep 2018 In : Parasite Immunology. e12592

Article DOI: 10.1111/pim.12592


The anti-fecundity effect of 5-azacytidine (5-AzaC) on Schistosoma mansoni is linked to dis-regulation transcription, translation and stem cell activities

Geyer, K., Munshi, S. E., Vickers, M., Squance, M., Wilkinson, T., Berrar, D., Chaparro, C., Swain, M. & Hoffmann, K. 01 Aug 2018 In : International Journal for Parasitology: Drugs and Drug Resistance. 8, 2, p. 213-222 10 p.

Article DOI: 10.1016/j.ijpddr.2018.03.006


Methyl-CpG-binding (SmMBD2/3) and chromobox (SmCBX) proteins are required for neoblast proliferation and oviposition in the parasitic blood fluke Schistosoma mansoni

Geyer, K., Munshi, S. E., Whiteland, H., Fernandez-Fuentes, N., Phillips, D. & Hoffmann, K. 28 Jun 2018 In : PLOS Pathogens.

Article DOI: 10.1371/journal.ppat.1007107


Detection of Galba truncatula, Fasciola hepatica and Calicophoron daubneyi environmental DNA within water sources on pasture land, a future tool for fluke control?

Jones, R., Brophy, P., Davis, C., Davies, T., Emberson, H., Rees Stevens, P. & Williams, H. 08 Jun 2018 In : Parasites & Vectors. 11, 342

Article DOI: 10.1186/s13071-018-2928-z


Purification of native Sigma Class Glutathione Transferase from Fasciola hepatica

Duncan, J., Cutress, D., Morphew, R. & Brophy, P. 01 Jun 2018 In : Molecular and Biochemical Parasitology. 222, p. 45-50 6 p.

Article DOI: 10.1016/j.molbiopara.2018.04.006


Design, synthesis and anthelmintic activity of 7-keto-sempervirol analogues

Crusco, A., Bordoni, C., Chakroborty, A., Whatley, K. C. L., Whiteland, H., Westwell, A. D. & Hoffmann, K. 25 May 2018 In : European Journal of Medicinal Chemistry. 152, p. 87-100

Article DOI: 10.1016/j.ejmech.2018.04.032


Histone methylation changes are required for life cycle progression in the human parasite Schistosoma mansoni

Roquis, D., Taudt, A., Geyer, K., Padalino, G., Hoffmann, K., Holroyd, N., Berriman, M., Allaga, B., Chaparro, C., Grunau, C. & de Carvalho Augusto, R. 21 May 2018 In : PLOS Pathogens. 14, 5, 26 p., 1007066

Article DOI: 10.1371/journal.ppat.1007066



Rethinking Schistosomiasis Vaccine Development: Synthetic Vesicles

Egesa, M., Hoffmann, K., Hokke, C. H., Yazdanbakhsh, M. & Cose, S. 01 Dec 2017 In : Trends in Parasitology. 1671, 4 p.

Article DOI: 10.1016/


Neoclerodane Diterpenoids from Reehal Fatima, Teucrium yemense

Nur-e-Alam, M, Yousaf, M, Ahmed, S, Al-Sheddi, ES, Shah, I, Fazakerley, D, Bari, A, Ghabbour, HA, Threadgill, MD, Whatley, K, Hoffmann, K & Al-Rehaily, AJ 2017, ' Journal of Natural Products.

Article DOI: 10.1021/acs.jnatprod.7b00188


Saprotrophic proteomes of biotypes of the witches’ broom pathogen Moniliophthora perniciosa

Pierre, S, Griffith, G, Morphew, R, Mur, L & Scott, I 2017, Fungal Biology. Article

DOI: 10.1016/j.funbio.2017.05.004


The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni

Geyer, K, Niazi, U, Duval, D, Cosseau, C, Tomlinson, C, Chalmers, I, Swain, M, Cutress, D, Bickham-Wright, U, Munshi, SE, Grunau, C, Yoshino, TP & Hoffmann, K 2017, PLoS Neglected Tropical Diseases, vol 11, no. 5, e0005246.

Article DOI: 10.1371/journal.pntd.0005246


Whole genome analysis of a schistosomiasis-transmitting freshwater snail

Adema, CM, Hillier, LW, Jones, CS, Loker, ES, Knight, M, Minx, P, Oliveira, G, Raghavan, N, Shedlock, A, do Amaral, LR, Arican-Goktas, HD, Assis, JG, Baba, EH, Baron, OL, Bayne, CJ, Bickham-Wright, U, Biggar, KK, Blouin, M, Bonning, BC, Botka, C, Bridger, JM, Buckley, KM, Buddenborg, SK, Lima Caldeira, R, Carleton, J, Carvalho, OS, Castillo, MG, Chalmers, IW, Christensens, M, Clifton, S, Cosseau, C, Coustau, C, Cripps, RM, Cuesta-Astroz, Y, Cummins, SF, Di Stephano, L, Dinguirard, N, Duval, D, Emrich, S, Feschotte, C, Feyereisen, R, Fitzgerald, P, Fronick, C, Fulton, L, Galinier, R, Gava, SG, Geusz, M, Geyer, KK, Giraldo-Calderón, GI, de Souza Gomes, M, Gordy, MA, Gourbal, B, Grunau, C, Hanington, PC, Hoffmann, KF, Hughes, D, Humphries, J, Jackson, DJ, Jannotti-Passos, LK, De Jesus Jeremias, W, Jobling, S, Kamel, B, Kapusta, A, Kaur, S, Koene, JM, Kohn, AB, Lawson, D, Lawton, SP, Liang, D, Limpanont, Y, Liu, S, Lockyer, AE, Lovato, TL, Ludolf, F, Magrini, V, Mcmanus, DP, Medina, M, Misra, M, Mitta, G, Mkoji, GM, Montague, MJ, Montelongo, C, Moroz, LL, Munoz-Torres, MC, Niazi, U, Noble, LR, Oliveira, FS, Pais, FS, Papenfuss, AT, Peace, R, Pena, JJ, Pila, EA, Quelais, T, Raney, BJ, Rast, JP, Rollinson, D, Rosse, IC, Rotgans, B, Routledge, EJ, Ryan, KM, Scholte, LLS, Storey, KB, Swain, M, Tennessen, JA, Tomlinson, C, Trujillo, DL, Volpi, EV, Walker, AJ, Wang, T, Wannaporn, I, Warren, WC, Wu, X-J, Yoshino, TP, Yusuf, M, Zhang, S-M, Zhao, M & Wilson, RK 2017, Nature Communications, vol 8, 15451 (2017), pp. 15451.

Article DOI: 10.1038/ncomms15451


The prevalence and development of digenean parasites within their intermediate snail host, Galba truncatula, in a geographic area where the presence of Calicophoron daubneyi has recently been confirmed

Jones, R, Williams, H, Dalesman, S, Sinmidele, A, Thomas, RK & Brophy, P 2017, Veterinary Parasitology.

Article DOI: 10.1016/j.vetpar.2017.03.021


Objective assessment of dietary patterns by use of metabolic phenotyping: a randomised, controlled, crossover trial 

Garcia-Perez, I, Posma, JM, Gibson, R, Chambers, ES, Hanson, TH, Vestergaard, H, Hansen, T, Beckmann, M, Pedersen, O, Elliott, P, Stamler, J, Nicholson, JK, Draper, J, Mathers, JC, Holmes, E & Frost, G 2017, Lancet Diabetes and Endocrinology, vol 5, no. 3, pp. 184-195. Article DOI: 10.1016/S2213-8587(16)30419-3


(Epi)genetic Inheritance in Schistosoma mansoni: A Systems Approach

Cosseau, C, Wolkenhauer, O, Padalino, G, Geyer, K, Hoffmann, K & Grunau, C 2016, ' Trends in Parasitology.

Article DOI: 10.1016/


Upgrading short read animal genome assemblies to chromosome level using comparative genomics and a universal probe set

Damas, J, O'connor, R, Farré, M, Lenis, V, Martell, H, Mandawala, A, Fowler, K, Joseph, S, Swain, M, Griffin, D & Larkin, DM 2016, Genome Research, vol 27, pp. 875-884.

Article DOI: 10.1101/gr.213660.116


Challenges and priorities for modelling livestock health and pathogens in the context of climate change

Özkan, Ş, Vitali, A, Lacetera, N, Amon, B, Bannink, A, Bartley, DJ, Blanco-penedo, I, De Haas, Y, Dufrasne, I, Elliott, J, Eory, V, Fox, NJ, Garnsworthy, PC, Gengler, N, Hammami, H, Kyriazakis, I, Leclère, D, Lessire, F, Macleod, M, Robinson, TP, Ruete, A, Sandars, DL, Shrestha, S, Stott, AW, Twardy, S, Vanrobays, M-L, Ahmadi, BV, Weindl, I, Wheelhouse, N, Williams, AG, Williams, HW, Wilson, AJ, Østergaard, S & Kipling, RP 2016, Environmental Research, vol 151, pp. 130-144.

Article DOI: 10.1016/j.envres.2016.07.033


MetaPred2CS: a sequence-based meta-predictor for protein-protein interactions of prokaryotic two-component system proteins

Kara, A, Vickers, M, Swain, M, Whitworth, D & Fernandez-Fuentes, N 2016, Bioinformatics, vol 32, no. 21, pp. 3339-3341.

Article DOI: 10.1093/bioinformatics/btw403


Rumen fluke Calicophoron daubneyi on Welsh farms: Prevalence, risk factors, and observations on co-infection with Fasciola hepatica

Jones, R, Brophy, P, Mitchell, ES & Williams, H 2016 Parasitology, pp. 237-247.

Article DOI: 10.1017/S0031182016001797


MultiGrain/MAPPER: A distributed multiscale computing approach to modeling and simulating gene regulation networks

Mizeranschi, AE, Swain, MT, Scona, R, Fazilleau, Q, Bosak, B, Piontek, T, Kopta, P, Thompson, P & Dubitzky, W 2016 Future Generation Computer Systems, vol 63, pp. 1-14.

Article DOI: 10.1016/j.future.2016.04.002

Antelope Diseases - the Good, the Bad and the Ugly. in Antelope Conservation: From Diagnosis to Action.

Kock, R, Chardonnet, P & Risley, C 2016, Conservation Science and Practice, Wiley, pp. 108-135.


Exploring and Expanding the Fatty-Acid-Binding Protein Superfamily in Fasciola Species

Morphew, R, Wilkinson, T, MacKintosh, N, Jahndel, V, Paterson, S, McVeigh, P, Abbas Abidi, SM, Saifullah, K, Raman, M, Ravikumar , G, LaCourse, EJ, Maule, AG & Brophy, P 2016, Journal of Proteome Research, vol 15, no. 9, pp. 3308-3321.

Article DOI: 10.1021/acs.jproteome.6b00331


DISMISS: MeDIP-seq: High-throughput sequencing

Niazi, U, Geyer, K, Vickers, M, Hoffmann, K & Swain, M, , 2016, Software, OMICtools.


DISMISS: detection of stranded methylation in MeDIP-Seq data

Niazi, U, Geyer, KK, Vickers, MJ, Hoffmann, K & Swain, M 2016, BMC Bioinformatics, vol 17, 295.

Article DOI: 10.1186/s12859-016-1158-7


InteractoMIX: a suite of computational tools to exploit interactomes in biological and clinical research

Poglayen, D, Marín-López, MA, Bonet, J, Fornes, O, Garcia-Garcia, J, Planas-Iglesias, J, Segura, J, Oliva, B & Fernandez-Fuentes, N 2016, Biochemical Society Transactions, vol 44, no. 3, pp. 917-924. Article DOI: 10.1042/BST20150001


The effects of PPO activity on the proteome of ingested red clover and implications for improving the nutrition of grazing cattle 

Hart, E, Onime, LA, Davies, T, Morphew, R & Kingston-Smith, A 2016, Journal of Proteomics, vol 141, pp. 67-76.

Article DOI: 10.1016/j.jprot.2016.04.023


Calmodulin disruption impacts growth and motility in juvenile liver fluke

McCammick, E, McVeigh, P, McCusker, P, Timson, D, Morphew, R, Brophy, P, Marks, N, Mousley, A & Maule, A 2016, ' Parasites & Vectors, vol 9, 46.

Article DOI: 10.1186/s13071-016-1324-9